A trend towards reduction of clinical scores was observed in EAE mice on long-term prolactin or combination treatment. (A) Mean clinical EAE scores in animals treated from Tx days 9 to 33 (bars indicate SEM). (B) Sum of EAE scores from Tx days 9 to 33. Statistical significance was not found although there was a tendency for the prolactin-alone, or combination treatment group to have lower clinical disease scores. There were seven mice each in the vehicle, prolactin-alone, or combination group, while there were five animals in the IFN-β-alone group.