The time course effects of CCI on spinal phospho-PTEN, PTEN, and phospho-mTOR. Spinal cord sections (10 μm) were from the sham-operated (A, D, G), 7 days after CCI (B, E, H), and 14 days after CCI (C, F, I) groups. Immunostaining images show cells labeled with phospho-PTEN (red, (A-C)), PTEN (green, (D-F)), and phospho-mTOR (red, (G-I)) in the spinal cord. Quantification of phospho-PTEN (J), PTEN (K), and phospho-mTOR (L) immunoreactivity in the ipsilateral dorsal horn of the lumbar spinal gray matter 1, 3, 7, and 14 days after CCI as compared with sham-operated rats. Each bar in panels (J-L) represents the mean ± SEM; six rats per group. CCI significantly upregulated spinal immunoreactivity of phospho-PTEN and phospho-mTOR and downregulated spinal PTEN immunoreactivity. CCI, chronic constriction injury; p-mTOR, phosphorylated mammalian target of rapamycin; PTEN, phosphatase and tensin homolog deleted from chromosome 10; p-PTEN, phosphorylated PTEN. Scale bars: 200 μm for all images (A-I). *P < 0.05 compared with the sham-operated group.