Time course of the effects of i.t. Ad-antisense PTEN on nociceptive behaviors in normal rats. The naïve rats exhibited mechanical allodynia (B) but not thermal hyperalgesia (A) and cold allodynia (C) from 3 to 7 days after i.t. injection of Ad-antisense PTEN. These data indicate the importance of downregulation of spinal PTEN for nociception. Each point represents the mean ± SEM with six rats per group. *P < 0.05 compared with vehicle group. Ad-GFP, adenovirus-mediated green fluorescent protein; Ad-PTEN, adenovirus-mediated phosphatase and tensin homolog deleted from chromosome 10; Ad-antisense PTEN, adenovirus-mediated PTEN antisense oligonucleotide.