Insulin improves memory and reduces chronic neuroinflammation in the hippocampus of young but not aged brains

Table 1 Primer sequences for gene expression analyses

Gene	Accession number	Primer sequences F: forward; R: reverse	Annealing temp (°C)	Product length
IL1ß	NM_031512	F: ACCTGCTAGTGTGTGATGTTCCCA	59.9	109
IL1ß	NM_031512	R: AGGTGGAGAGCTTTCAGCTCACAT	59.9	109
TNFα	X66539.1	F: CTGGCCAATGGCATGGATCTCAAA	60	97
TNFα	X66539.1	R: AGCCTTGTCCCTTGAAGAGAACCT	60	97
GADPH	NM_017008	F: TGACTCTACCCACGGCAAGTTCAA	59.9	141
GADPH	NM_017008	R: ACGACATACTCAGCACCAGCATCA	60	141
CREB1	NM_134443	F: TGTTCAAGCTGCCTCTGGTGATGTA	60	141
CREB1	NM_134443	R: ACCTCTCTCTTTCGTGCTGCTTCTT	60	141
BDNF	BC087634	F: AGCCTGTGTACTTTGTGTCCGAGA	60.3	123
BDNF	BC087634	R: TGGACGTTTGCTTCTTTCATGGGC	60.3	123
IR	NM_017071	F: AGACCTTCGAGGATTACCTGCACAAC	60.3	118
IR	NM_017071	R: TTGCCCACCTCTTCAAGGGATCTT	60.4	118
PRKCζ	NM_022507	F:AGAGGGATCATCTACCGGGACCTAA	60.2	85
PRKCζ	NM_022507	R:ACCTCTCTCTTTCGTGCTGCTTCTT	60.1	85
NGF β	NM_001277055	F: ACTTCCAGGCCCATGGTACAATCT	60.2	145
NGF β	NM_001277055	R: ATGTCCGTGGCTGTGGTCTTATCT	60	145
CAMKIIα	NM_012920	F: ACCAACACCACCATCGAGGATGAA	60.4	135
CAMKIIα	NM_012920	R: TGTCATTCCAGGGTCGCACATCTT	60.6	135
PSD95	NM_019621	F:AGCGACGAGAGTGGTCAAGGTTAAAG	60	139
PSD95	NM_019621	R:CCAAGGATGATGATGGGACGAGCATA	60	139
SYN1	NM_019133	F: TCTCCAGCACACCTTGCTCCTAATC	60	128
SYN1	NM_019133	R:CTGGTTGGTGGATAGTAGGTGGCATTT	60	128

BDNF, brain-derived neurotrophic factor; CaMKIIα, Ca2+/calmodulin-dependent protein kinase II-alpha; CREB1, cAMP response element-binding protein-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; IL, interleukin; iNOS, inducible nitric oxide synthase; IR, insulin receptor; NGF β, nerve growth factor-beta; PRKCζ, protein kinase C-zeta; PSD95, postsynaptic density protein 95; SYN1, Synapsin I; TNF-α, tumor necrosis factor-alpha.

ISSN: 1742-2094