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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Sterile alpha motif and histidine/aspartic acid domain-containing protein 1 (SAMHD1)-facilitated HIV restriction in astrocytes is regulated by miRNA-181a

Figure 4

miR-155/-181a inhibition increases HIV replication. (A) Astrocytes (2 × 105 cells/ml) were transiently transfected with mimic or inhibitor for miR-155, miR-181a, and miR-146a, at a final concentration of 50 nmol, followed by HIV infection. After 72 h, supernatants were collected and assayed for p24 by ELISA. (B) Astrocytes (2 × 105 cells/ml) were transiently transfected with miR-181a mimic, followed by infection with HIV-1BAL (100 ng p24) with or without 100 nm AZTTP. p24 level was measured in the supernatant from astrocytes cultures. (C) Astrocytes (2 × 105 cells/ml) were transiently transfected with SAMHD1 siRNA (75 pmols) and miR-181a inhibitor (50 nmols) individually or a combined transfection with both. After 72 h, supernatants were collected and assayed for p24 by ELISA. Results shown are representative of mean ± SEM of three independent experiments. P value ≤0.05 were considered to be significant. SAMHD1, sterile alpha motif and histidine/aspartic acid domain-containing protein 1; AZTTP, azidothymidine triphosphate.

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