Figure 1

IVIg treatment inhibits the increased expressions of TLRs in cultured cortical neurons subjected to simulated ischemia. (A-D) Representative 2immunoblots and quantification illustrating increases in the levels of TLR2, TLR4 and TLR8 proteins in primary cortical neurons at indicated times during GD. Administration of IVIg (5 and 10 mg/ml) significantly prevents GD-induced increased expression of TLR2, TLR4 and TLR8. Data are represented as mean ± SEM. n = 5 cultures. ^* P < 0.05 in comparison with normal; ^** P < 0.01 in comparison with normal; ^*** P < 0.001 in comparison with normal; ⁺⁺⁺ P < 0.001 in comparison with vehicle-treated group. (E-H) Representative immunoblots and quantification illustrating increases in the levels of TLR2, TLR4 and TLR8 proteins in primary cortical neurons at indicated times during OGD. Administration of IVIg (5 and 10 mg/ml) significantly prevents OGD-induced increased expression of TLR2, TLR4 and TLR8. Data are represented as mean ± SEM. n = 5 cultures. ^** P < 0.01 in comparison with normal; ^*** P < 0.001 in comparison with normal; ⁺ P < 0.05 in comparison with vehicle-treated group; ⁺⁺ P < 0.01 in comparison with vehicle-treated group; ⁺⁺⁺ P < 0.001 in comparison with vehicle-treated group.