Figure 2

IVIg treatment prevents the increased expressions of TLR adaptor and signalling proteins. IVIg treatment prevents the increased expressions of TLR adaptor and signalling proteins in cultured cortical neurons subjected to simulated ischemia. (A-C) Representative immunoblots and quantification illustrating increases in the levels of MyD88 and TRAF6 proteins in primary cortical neurons at indicated times during GD. Administration of 10 mg/ml of IVIg significantly prevents the increase in MyD88 and TRAF6 protein expression caused by 6, 12 and 24 h of GD; the effect of 5 mg/ml IVIg was significant at 12 and 24, but not 6 h of GD. Data are represented as mean ± SEM. n = 5 cultures. ^** P < 0.01 in comparison with normal; ^*** P < 0.001 in comparison with normal; ⁺⁺⁺ P < 0.001 in comparison with vehicle-treated group; ns, not significant. (D-F) Representative immunoblots and quantification illustrating increases in the levels of MyD88 and TRAF6 proteins in primary cortical neurons at indicated times during OGD. Administration of IVIg (5 and 10 mg/ml) significantly prevents an increased expression level of MyD88 and TRAF6 proteins following 6 and 12 h of OGD. Data are represented as mean ± SEM. n = 5 cultures. ^* P < 0.05 in comparison with normal; ^*** P < 0.001 in comparison with normal; ⁺⁺ P < 0.01 in comparison with vehicle-treated group; ⁺⁺⁺ P < 0.001 in comparison with vehicle-treated group.