Figure 4

IVIg protects cultured neurons against simulated ischemia. (A, B) Treatment with high concentrations of IVIg (5 and 10 mg/ml) significantly prevents GD-induced increased expression of pro-apoptotic cleaved caspase-3 following 6, 12 and 24 h. Data are represented as mean ± SEM. n = 5 cultures. ^*** P < 0.001 in comparison with normal; ⁺ P < 0.05 in comparison with vehicle-treated group; ⁺⁺ P < 0.01 in comparison with vehicle-treated group; ⁺⁺⁺ P < 0.001 in comparison with vehicle-treated group. (A, C) Treatment with high concentrations of IVIg (5 and 10 mg/mL) significantly increased the expression level of anti-apoptotic Bcl-2 following 6, 12 and 24 h of GD. Data are represented as mean ± SEM. n = 5 cultures. ⁺ P < 0.05 in comparison with vehicle-treated group; ⁺⁺⁺ P < 0.001 in comparison with vehicle-treated group; ns, not significant. (D, E) Treatment with high concentrations of IVIg (5 and 10 mg/mL) significantly prevents OGD-induced increased expression of pro-apoptotic cleaved caspase-3 following 6 and 12 h. Data are represented as mean ± SEM. n = 5 cultures. ^** P < 0.01 in comparison with normal; ^*** P < 0.001 in comparison with normal; ⁺ P < 0.05 in comparison with vehicle-treated group; ⁺⁺ P < 0.01 in comparison with vehicle-treated group; ⁺⁺⁺ P < 0.001 in comparison with vehicle-treated group. (D, F) Treatment with high concentrations of IVIg (5 and 10 mg/mL) significantly prevents a decreased level of anti-apoptotic Bcl-2 following 6 and 12 h of OGD. Data are represented as mean ± SEM. n = 5 cultures. ^** P < 0.01 in comparison with normal; ⁺⁺⁺ P < 0.001 in comparison with vehicle-treated group.