Figure 5

IVIg treatment prevents the increased expression of TLR signalling components in vivo following stroke. (A-F) Representative immunoblots and quantification illustrating increases in the levels of TLR2, TLR4 and TLR8 proteins and both TLR adaptor protein MyD88 and signalling protein TRAF6 in ipsilateral brain tissues of C57BL/6 J mice following MCAO (1 h) and reperfusion (6 and 24 h). Administration of IVIg (1 g/kg) significantly prevents the increased expression levels of TLR2, TLR4 and TLR8 as well as MyD88 following 6 and 24 h of cerebral ischemia and reperfusion (I/R). However, administration of IVIg (1 g/kg) only significantly prevents the increased expression level of TRAF6 in mice subjected to 6 h of cerebral I/R, not in mice subjected to 24 h of cerebral I/R. Data are represented as mean ± SEM. n = 5 to 6 animals in each group. ^## P < 0.01 in comparison with sham; ^### P < 0.001 in comparison with sham; *P < 0.05 in comparison with I/R 6; **P < 0.01 in comparison with I/R 6; ***P < 0.001 in comparison with I/R 6; ⁺ P < 0.05 in comparison with I/R 24; ⁺⁺⁺ P < 0.001 in comparison with I/R24; ns, not significant.