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Figure 1 | Journal of Neuroinflammation

Figure 1

From: Activated microglia/macrophage whey acidic protein (AMWAP) inhibits NFκB signaling and induces a neuroprotective phenotype in microglia

Figure 1

AMWAP is secreted from reactive BV-2 microglia. (A) BV-2 microglial cells were treated with 50 ng/ml LPS for 48 h before obtaining cellular protein lysates (L) as well as concentrated culture supernatants (S). Anti-AMWAP immunoblot analysis revealed a band of approximately 12 kDa only present in the supernatant of LPS-activated microglia. GAPDH and Ponceau S served as loading controls. (B) Anti-AMWAP immunoblot analysis of recombinantly expressed and purified AMWAP from E. coli (AMWAP-His) and HEK293 EBNA cells (AMWAP-Strep(II)) shows single bands at approximately 11 to 13 kDa.

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Journal of Neuroinflammation

ISSN: 1742-2094