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Figure 10 | Journal of Neuroinflammation

Figure 10

From: Activated microglia/macrophage whey acidic protein (AMWAP) inhibits NFκB signaling and induces a neuroprotective phenotype in microglia

Figure 10

AMWAP promotes microglial filopodia formation and phagocytosis. (A) Representative images of Phalloidin-TRITC labeled BV-2 microglial cells pre-treated with 10 μg/ml AMWAP or vehicle for 24 h in the absence or presence of 50 ng/ml LPS for 24 h. (B) CFSE-proliferation assay of BV-2 microglia treated with 10 μg/ml AMWAP or vehicle for 24 h in the absence or presence of 50 ng/ml LPS for 24 h. The proliferation rate was assessed 24 h after LPS treatment using flow cytometry and a representative graph out of three repetitions is shown. (C, D) Phagocytosis of CM-DiI-stained apoptotic 661W photoreceptor debris by BV-2 microglia was monitored and quantified after 6-h feeding time of AMWAP- vs. vehicle-treated cells in the absence or presence of 50 ng/ml LPS for 24 h. Data show mean ± SD (n = 9/group) with *P < 0.05, **P < 0.01, ***P < 0.001 for AMWAP-treated cells vs. control and AMWAP + LPS vs. LPS-treated cells, respectively. PBS served as a vehicle control. CFSE, carboxyfluorescein diacetate succinimidyl ester. Scale bar = 20 μm.

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