Figure 2

AMWAP reduces pro-inflammatory marker gene transcription in BV-2 microglia. BV-2 cells were treated with various concentrations of recombinant AMWAP for 24 h before further stimulation with 50 ng/ml LPS for 12 h. Transcript levels of the pro-inflammatory markers IL6 (A), iNOS (B), CCL2 (C), and CASP11 (D) were determined by quantitative real-time PCR. Data show mean ± SD (n = 3/group, measured in triplicate) with *P < 0.05, **P < 0.01, ***P < 0.001 for AMWAP + LPS- vs. LPS-treated cells. PBS served as vehicle control.