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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Activated microglia/macrophage whey acidic protein (AMWAP) inhibits NFκB signaling and induces a neuroprotective phenotype in microglia

Figure 3

AMWAP reduces TLR4-mediated pro-inflammatory gene transcription in embryonic stem cell-derived microglia and primary brain microglia. (A-E) Embryonic stem cell-derived microglia (ESdM) were treated with 10 μg/ml of recombinant AMWAP for 24 h before stimulation with 500 ng/ml LPS for further 24 h. (F-J) Primary brain microglia were treated with 10 μg/ml of recombinant AMWAP for 24 h before stimulation with 50 ng/ml LPS for further 24 h. mRNA expression of the pro-inflammatory marker transcripts IL6 (A, F), iNOS (B, G) CCL2 (C, H), CASP11 (D, I), and TNFα (E, J) was determined by quantitative real-time RT-PCR. Data show mean ± SD (n = 3/group, measured in triplicate) with *P < 0.05, **P < 0.01, ***P < 0.001 for AMWAP + LPS- vs. LPS-treated cells. PBS served as vehicle control.

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Journal of Neuroinflammation

ISSN: 1742-2094