Figure 4

AMWAP reduces TLR2-mediated pro-inflammatory gene transcription in BV-2 microglia. BV-2 cells were treated with 10 μg/ml of recombinant AMWAP for 24 h before stimulation with 50 μg/ml zymosan (A-E) or 4 μg/ml CpG oligodeoxynucleotides (F-J) for additional 24 h. The pro-inflammatory marker transcripts IL6 (A, F), iNOS (B, G), CCL2 (C, H), CASP11 (D, I), and TNFα (E, J) were measured by quantitative real-time RT-PCR. Data show mean ± SD (n = 3/group, measured in triplicate) with *P < 0.05, **P < 0.01, ***P < 0.001 for AMWAP + zymosan/CpG- vs. zymosan/CpG-treated cells. PBS served as vehicle control.