Immunoprecipitation analysis showing eNOS/HSP90 complex formation (as in Figure 2) in response to stimulation with 20 ng/ml VEGF of BAECs cultured in the presence of Aβ25–35
(A) and 1 μM Aβ1–42
(B) for 24 h and in combination with 2 mM NAC. (C-D) The blots were subjected to densitometric analysis, and the obtained data were analyzed for statistical significance in comparison to the data showed in Figure 2A,B. (*P < 0.05 versus 0 min of each separate treatment group and #P < 0.02 versus 0 min of untreated control cells (white bars), n = 4). VEGF, vascular endothelial growth factor; eNOS, endothelial nitric oxide synthase; min, minutes; NAC, N-acetyl-cysteine; Hsp90, heat shock protein 90.