Representative Western blotting showing phosphorylation of Akt at serine 473 in response to stimulation with 20 ng/ml VEGF of BAECs cultured for 24 h in the presence of 10 μM Aβ25–35
(A) or 5 μM Aβ1–42
(B) in combination with 2 mM NAC. (C-D) The blots were subjected to densitometric analysis, and the obtained data were analyzed for statistical significance in comparison to the data shown in Figure 2A,B. (*P < 0.05 versus 0 min of each separate treatment group, n = 3). VEGF, vascular endothelial growth factor; eNOS, endothelial nitric oxide synthase; min, minutes; NAC, N-acetyl-cysteine.