JZL184 decreased secretion of NO and IL-1β from adult glial cells exposed to proinflammatory agents. Astrocytes and microglia were pre-incubated with MAGL inhibitor JZL184 for 30 min before inducing proinflammatory responses by simultaneous exposure of 1 μM LPS and 100 ng/ml IFN-γ. After 24 h of incubation, secretion of NO was reduced (A) slightly in microglia and (B) significantly (P < 0.01) in astrocytes treated with JZL184 compared to exposed cells w/o JZL184 treatment. JZL184 induced (C) a marked reduction in IL-1β secretion from microglia (P < 0.001) but (D) did not have an effect on astrocytes compared to cells w/o JZL184 treatment. Pre-treatment with JZL184 decreased (E) Iba1 and (F) GFAP expression slightly in microglia and astrocytes induced with LPS and IFN-γ and significantly in microglia and astrocytes induced with Aβ42 (P < 0.01).