Figure 7

COX-2 inhibition by biphenyl neolignans in different biological matrices. (A-D) 27a, (E-H) 32, and (I-L) 34a inhibition of 2-AG, AEA, and AA oxygenation in hCOX-2 (A, E, I), RAW264.7 cell homogenates (B, F, J), and intact cells (C, G, K) incubated with 10 μM of substrate (2-AG, AEA, or AA depending on the assay). (D, H, L) Inhibition of PGE₂ and PGE₂-GE formation in RAW264.7 cells upon LPS (1 mg mL⁻¹) ATP (1 mM) and thapsigargin (2 μM) treatment without externally adding 2-AG. ^* P < 0.05, ^** P < 0.01 PGE₂-GE vs. PGE₂.