Hematoxylin and eosin staining of retinal sections. (A) WT control animals reveal clearly defined nuclear and plexiform layers. (B) WT hypo mouse retinas reveal thin and highly disorganized cell and plexiform layers with numerous pyknotic cells (arrow).The ganglion cell layer appear thin and is populated by small cell bodies. The smaller picture shows a central section of a WT hypo specimen with almost no survival. (C) KO control mice display clearly defined nuclear and plexiform layers. (D) Hypoperfused Gal-3 knockout mouse retinas (KO hypo) show a clearly laminated architecture with well populated cell layers. Scale bar = 50 μm.