Immunohistochemical labeling of Gal-3, with Müller cell markers GFAP and CRALBP, and DAPI; epifluorescent imaging and confocal imaging of WT hypoperfused retinas. (A) A confocal linescan z-stack image of a double labeling of Gal-3/CRALBP show Gal-3 labeling of large structures vertically spanning the inner layers (arrow), as well as scattered structures. No colocalized labeling of CRALBP and Gal-3 was found. (B) Confocal image of Gal-3/CRALBP double labeling displays no colocalization. Large, vertical Gal-3 positive structures are present (arrow). C) Gal-3/GFAP double labeling reveals no colocalization. Scale bars = 50 μm.