Immunohistochemical labeling of ganglion cells. (A) WT control retinas reveal large cell bodies of ganglion morphology in the GCL. (B) WT hypo mice show only isolated cell bodies in the GCL. (C) KO control animals show plentiful large cell bodies of ganglion morphology in the GCL. (D) KO hypo retinas reveal large cell bodies in the GCL. Scale bar = 50 μm. (E) Statistical analysis of NeuN-labeled ganglion cells in the GCL. No significant difference between WT and KO control animals. Significantly fewer ganglion cells in WT hypo animals compared to corresponding controls and KO hypo retinas (P < 0.001; P < 0.05, respectively). Error bars SEM. *P < 0.05; **P < 0.01; ***P < 0.001.