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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Who let the dogs out?: detrimental role of Galectin-3 in hypoperfusion-induced retinal degeneration

Figure 5

Immunohistochemical labeling of bipolar cells (PKCpan), horizontal cells (Calbindin). A-D PKCpan. E-H Calbindin. (A) WT control animals show numerous well organized bipolar cells and their processes in the INL and IPL, with strong labeling intensity present in the cell bodies in the INL. (B) WT hypo retinas display short bipolar cells with disorganized cell bodies and terminals and sprouting processes in the ONL (arrows). (C) KO control retinas show a multitude of well organized bipolar cells and their processes in the INL and IPL, respectively. Strong labeling is present in the terminals in the IPL and the cell bodies in the INL. (D) KO hypo specimens display numerous bipolar cells and processes in the INL and IPL, with retained organization and no sprouting. (E) WT control retinas reveal horizontal cell bodies and their processes in the INL and OPL, respectively. (F) WT hypo animals, no labeled cells are seen; however, scattered labeling of unidentified structures is present. (G) KO control animals display horizontal cell bodies and their processes in the INL and OPL. (H) KO hypo specimens reveal horizontal cell bodies and their processes in the INL and OPL. Scale bars = 50 μm. (I) Statistical analysis of PKCpan width (bipolar cell length) in the INL and OPL. Significantly shorter bipolar cells in WT hypo animals compared to corresponding controls and KO hypo retinas (P < 0.001 for both comparisons). Error bars SEM. *P < 0.05; **P < 0.01; ***P < 0.001.

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