Skip to main content

Advertisement

Table 1 Table of primary and secondary antibodies used for immunohistochemical analysis

From: Who let the dogs out?: detrimental role of Galectin-3 in hypoperfusion-induced retinal degeneration

  Antibody name Target cell Species Dilution Source
Antigen      
 Neuronal nuclei (NeuN) Anti-neuronal nuclei Ganglion cells Mouse monoclonal 1:100 Millipore, USA
 Glutamine synthetase (GS) Rabbit anti-GS Müller cells Rabbit polyclonal 1:200 Abcam, Cambridge, UK
 Recoverin Anti-recoverin Rod and cone photoreceptors Rabbit polyclonal 1:10,000 Chemicon International, CA, USA
 Rhodopsin Rho4D2 Rod photoreceptor Mouse monoclonal 1:100 Kind gift of Prof. RS Molday, Vancouver, Canada
 Iba-1 Rabbit anti-Iba-1 Microglia Rabbit polyclonal 1:500 Wako Pure Chemical Industries Ltd., Osaka, Japan/Nordic biolabs
 Galectin-3 Galectin-3 antibody Mainly microglia Rat 1:300 Hakon Leffler
 Calbindin Anti-calbindin D-28K Horizontal cells Mouse monoclonal 1:200 Sigma Aldrich, St Louis, MO, USA
 Glial fibrillary acidic protein (GFAP) Anti-glial fibrillary acidic protein Activated Müller cells, astrocytes Mouse monoclonal 1:200 Chemicon International, CA, USA
 PKCpan Phospho-PKC (pan) Rod bipolar cells Rabbit polyclonal 1:200 Cell Signaling, Beverly, MA, USA
 Cellular retinaldehyde-binding protein (CRALBP) Anti-CRALBP [B2] Müller cells Mouse monoclonal 1:200 Chemicon International, CA, USA
Secondary antibody      
 Fluorescein isothiocyanate (FITC) Anti-mouse IgG FITC conjugate Anti-mouse Goat 1:200 Sigma Aldrich, St Louis, MO, USA
 Texas red Texas red dye-conjugated AffiniPure Anti-rabbit Donkey 1:200 Jackson ImmunoResearch, PA, USA