Table 1 Table of primary and secondary antibodies used for immunohistochemical analysis
From: Who let the dogs out?: detrimental role of Galectin-3 in hypoperfusion-induced retinal degeneration
| Antibody name | Target cell | Species | Dilution | Source | |
|---|---|---|---|---|---|
| Antigen | |||||
| Neuronal nuclei (NeuN) | Anti-neuronal nuclei | Ganglion cells | Mouse monoclonal | 1:100 | Millipore, USA |
| Glutamine synthetase (GS) | Rabbit anti-GS | Müller cells | Rabbit polyclonal | 1:200 | Abcam, Cambridge, UK |
| Recoverin | Anti-recoverin | Rod and cone photoreceptors | Rabbit polyclonal | 1:10,000 | Chemicon International, CA, USA |
| Rhodopsin | Rho4D2 | Rod photoreceptor | Mouse monoclonal | 1:100 | Kind gift of Prof. RS Molday, Vancouver, Canada |
| Iba-1 | Rabbit anti-Iba-1 | Microglia | Rabbit polyclonal | 1:500 | Wako Pure Chemical Industries Ltd., Osaka, Japan/Nordic biolabs |
| Galectin-3 | Galectin-3 antibody | Mainly microglia | Rat | 1:300 | Hakon Leffler |
| Calbindin | Anti-calbindin D-28K | Horizontal cells | Mouse monoclonal | 1:200 | Sigma Aldrich, St Louis, MO, USA |
| Glial fibrillary acidic protein (GFAP) | Anti-glial fibrillary acidic protein | Activated Müller cells, astrocytes | Mouse monoclonal | 1:200 | Chemicon International, CA, USA |
| PKCpan | Phospho-PKC (pan) | Rod bipolar cells | Rabbit polyclonal | 1:200 | Cell Signaling, Beverly, MA, USA |
| Cellular retinaldehyde-binding protein (CRALBP) | Anti-CRALBP [B2] | Müller cells | Mouse monoclonal | 1:200 | Chemicon International, CA, USA |
| Secondary antibody | |||||
| Fluorescein isothiocyanate (FITC) | Anti-mouse IgG FITC conjugate | Anti-mouse | Goat | 1:200 | Sigma Aldrich, St Louis, MO, USA |
| Texas red | Texas red dye-conjugated AffiniPure | Anti-rabbit | Donkey | 1:200 | Jackson ImmunoResearch, PA, USA |