Figure 2
From: Autoantigen cross-reactive environmental antigen can trigger multiple sclerosis-like disease
Induction of autoimmune paralysis in TCR transgenic multiple sclerosis model by a cross-reactive Dictyostelium slime mold peptide. Previously described HLA-DR15 and TCR transgenics carrying an anti-MBP (85-99) specific TCR (clone Ob1.A12) and HLA-DR1501, Ao were used to test the encephalitogenic capacity of test peptides. Young male mice received 300 μg peptide subcutaneously in supplemented CFA at day 0 and 50 μg peptide subcutaneously in CFA at day 6. Pertussis toxin at a dose of 200 ng intraperitoneally was administered on days 0, 2, 6, and 8. Mice were scored daily for signs of neurological disease using an established disease score scale according to the following criteria: 0, normal; 1, limp tail; 2, impaired righting reflex or waddling gait; 3, partial hind limb paralysis; 4, total hind limb paralysis; 5, total limb paralysis. HLA-DR15 and TCR transgenics carrying the anti-MBP (85-99)-specific TCR (clone Ob1.A12) and HLA-DR1501, Ao were administered MBP 85-99 peptide (closed circles, n = 6), Dictyostelium fasciculatum YKHKIHFFKNEVLE peptide (closed squares, n = 6), Emiliania huxleyi CCMP1516 STARVHFWRSRSSE peptide (closed triangles, n = 6), control peptide, Burkholderia pseudomallei AhpC (51-70) KDFTFVCPTEIVEFAKLAKQ (closed diamonds, n = 6), C57BL/6 mice administered MBP 85-99 peptide (open circles, n = 5), Emiliania huxleyi CCMP1516 STARVHFWRSRSSE peptide (open triangles, n = 5), Dictyostelium fasciculatum YKHKIHFFKNEVLE peptide (open squares, n = 5), and Burkholderia pseudomallei AhpC (51-70) KDFTFVCPTEIVEFAKLAKQ (open diamonds, n = 5). MBP, myelin basic protein.