Figure 4

Effect of aging on SVZ cell proliferation. (A) Number of BrdU+ cells in the SVZ at 7 and 14 days after MCAO in young and aged mice. Data are mean ± SD, n = 6 to 8. (B) Correlation between number of BrdU+ cells in SVZ at 7 days and infarct size from aged mice (n = 10). (C) Diagram with SVZ cell markers. (D) Scheme. (E) Cytometric quantification of prominin-1⁺/EGFR⁺/nestin⁺ cell population (right upper) and of type-B cells (GFAP⁺ cells; right lower). (F) Quantification of prominin-1⁺/EGFR⁺/nestin⁻ cell population (left upper) and of type-C cells (Mash1+ cells; left lower). SVZ cells were obtained after MCAO from young and aged mice. Cell suspensions were labeled with antibodies for the different markers and analyzed by flow cytometry (see Methods for details). Data are mean ± SD, n = 6 to 8, *P < 0.05. MCAO, middle cerebral artery occlusion; EGFR, epidermal growth factor receptor; GFAP, glial fibrillary acidic protein; SVZ, subventricular zone.