Table 2 Immunohistochemistry: primary antibodies
Antigen | Primary Antibody | Source | Dilution |
|---|---|---|---|
Glial fibrillary acidic protein (GFAP) | Polyclonal rabbit | DAKO, Glostrup, Denmark | 1:4,000 |
Neuronal nuclear protein (NeuN) | Mouse clone MAB377 | Chemicon, Temecula, CA, USA | 1:2,000 |
Synaptophysin | Mouse clone Sy38 | DAKO Glostrup, Denmark | 1:200 |
Ki67 | Mouse clone MIB-1 | DAKO, Glostrup, Denmark | 1:200 |
BRAF V600E | VE1 clone | –a | 1:5 |
CD34 | Mouse clone QBEnd10 | Immunotech, Marseille, Cedex, France | 1:600 |
Phospho-S6 ribosomal protein (pS6) | Monoclonal rabbit (Ser235/236) | Cell Signaling Technology, Beverly, MA, USA | 1:200 |
Human leukocyte antigen (HLA-DP, DQ, DR; MHC-II) | Mouse clone CR3/43 | DAKO, Glostrup, Denmark | 1:100 |
IL-1β | Polyclonal goat antibody | Santa Cruz Bio., CA, USA | 1:70 |
IRAK1 | Mouse clone 3 F7 | Sigma, St. Louis, Mo, USA, | 1:300 |
TRAF6 | Monoclonal rabbit | Abcam, Cambridge, MA, USA | 1:300 |
PDCD4 | Polyclonal rabbit | Abcam, Cambridge, MA, USA | 1:450 |
SHIP1 | Polyclonal rabbit | Cell Signaling, Beverly, MA, USA | 1:300 |