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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: CXCR2 is essential for cerebral endothelial activation and leukocyte recruitment during neuroinflammation

Fig. 2

Chemokine levels and the effect of CXCR2 or CXCL1 deficiency on neutrophil recruitment to the brain parenchyma after i.c.v. LPS injection. Wild-type mice were i.c.v. injected with LPS. CXCL1 (A), CXCL2 (B), and CXCL5 (C) concentrations in the brains of WT (C57BL/6J) mice were determined via ELISA at various time points after i.c.v. LPS injection. WT mice i.c.v. injected with saline for 24 h served as negative controls. Infiltrating neutrophils in the cortex (D) and hippocampus (E) were quantified via esterase staining of the brain sections 4, 12, 24, or 48 h after i.c.v. LPS or saline injection. (F) The WT, CXCR2−/−, and CXCL1 mice were i.c.v. injected with LPS 24 h before the quantification of infiltrating neutrophils. Representative photomicrographs of brain sections stained for esterase positive neutrophils (arrows) from wild-type, (G–I) CXCR2−/− and CXCL1 mice. Scale bar: 100 μm; 10 μm (inset). CXCR2 and CXCL1 deficiency significantly reduced neutrophil recruitment in the cortex (G), hippocampus (H), and choroid plexus (I). The results are presented as the means ± SEM and represent a minimum of five mice per group. *P < 0.05; **P < 0.01

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