Fig. 4

The effect of CXCR2 or CXCL1 deficiency on the mRNA expression of adhesion molecules in vivo. The mRNA expression of P-selectin, E-selectin, VCAM-1, and ICAM-1 saline-treated (4 h after i.c.v. saline injection) control group of WT mice and LPS-treated (4 h after i.c.v. LPS injection) WT, CXCR2^−/−, and CXCL1^−/− mice was quantified via real-time PCR. Both CXCR2 and CXCL1 deficiency resulted in the down-regulation of P-selectin (A), E-selectin (B), VCAM-1 (C), and ICAM-1 (D) mRNA expression in the brain. n = 6–8 mice for all groups. *P < 0.05; **P < 0.01