Fig. 8

The effect of CXCR2 antagonist infusion on leukocyte rolling and adhesion in CNS vessels. WT mice received an intravenous injection of the CXCR2 antagonist SB225002 (1 mg/kg) 0.5 h prior to i.c.v. LPS injection. Four hours after i.c.v. LPS injection, the protein expression of P-selectin, VCAM-1, and E-selectin in the brain was determined by Western blot analysis (A). Intravital microscopy was performed on the mice. The results of leukocyte recruitment (B) are presented as the mean ± SEM. n = 4 mice for all groups. *P < 0.05; **P < 0.01