Fig. 1
Aβ-specific CD4+ T cells and antibodies but not CD8+ T cells are triggered in HLA-A2.1/HLA-DR1 mice upon Aβ vaccination. (a) Frequency of IFN-γ secreting splenocytes in Aβ-immunized HLA-A2.1/HLA-DR1 mice, as assessed by ELISPOT. Splenocytes (106/wells) from mice immunized with either PBS/CpG/IFA or Aβ1-42 in CpG/IFA were stimulated in triplicate for 18 h at 37 °C with Aβ1-42 (40 μg/ml) or 15-mer overlapping peptides (10 μg/ml) covering the full length of Aβ1-42. Results are presented as numbers of peptide-specific IFNγ-secreting cells per 106 splenocytes, calculated after subtracting the mean number of spots obtained in the absence of peptide. (b) Serum levels of Aβ-specific antibodies were measured by ELISA using Aβ-coated plates. Data are expressed as relative OD. Mean ± SD (two to four mice/group). Results are representative of two independent experiments