Fig. 8

Aβ33-41NP specifically promotes CD8⁺ T cell infiltration into the brain of APPPS1 mice. Four-month-old APPPS1 mice or WT littermates were immunized twice with 23-31NP or 33-41NP mixed in CpG/HBV/IFA, or treated with PBS/CpG/HBV/IFA alone. Brains were harvested between 6 and 7 month of age, and immune cell infiltration was analyzed by immunohistochemistry. Brain sections were stained with anti-CD3 + anti-CD8 mAbs or anti-CD11b mAb. (a) Representative images from brain cortex of control or 33-41NP-immunized APPPS1 mice. (b) Numbers of CD3⁺CD8⁻ or CD3⁺CD8⁺ cells per brain section. (c) CD8⁻/CD8⁺ ratio. (d) Percentage of CD8⁺ cells in the blood and spleen analyzed by flow cytometry. (e) Representative images of CD11b labeling in the cortex of control or 33-41NP-immunized APPPS1 mice. (f) Numbers of CD11b⁺ cells per brain section. Mean ± SEM (three to five mice/group). Results are representative of two independent experiments. Mann–Whitney U test, *p ≤ 0.05, **p ≤ 0.01