Fig. 5

Regulation of NF-κB in RAW264.7 and primary bone marrow macrophages. a Western blot showing serine 536 phosphorylation of p65 in LPS-stimulated RAW264.7 macrophages stably transduced with scrambled (black columns) or TBK1-specific shRNA (light grey columns), (n = 4). The blots show a representative result; the diagram shows the densitometric analysis of all experiments. b Western blot showing serine 536 phosphorylation of p65 in LPS-stimulated BMMs derived from TNFR^−/− (dark grey columns) and TBK1^−/−/TNFR^−/− (light grey columns) mice, (n = 4). The blots show a representative result, the diagram shows the densitometric analysis of all experiments. Univariate ANOVA with Bonferroni post-hoc analysis *P < 0.05 and ***P < 0.001 statistically significant differences compared with unstimulated cells. Student’s t test ^# P < 0.05 and ^## P < 0.01 significant mean difference between scrambled shRNA and TBK1 shRNA or TNFR^−/− and TBK1^−/−/TNFR^−/− cells, respectively