Fig. 6

Regulation of NF-κB dependent and independent genes in the spinal cord. Quantitative RT-PCR (Taqman) of cyclooxygenase-2 (COX-2), inducible NO-synthase (iNOS), matrix metalloproteinase-9 (MMP-9), c-fos, and Akt1 mRNA in the spinal cord of naïve mice and 2 h after formalin injection into a hind paw. GAPDH-RNA was used as reference control gene (n = 4 mice/group) and each Taqman-PCR was performed twice in triplicate; black columns = wild type; dark grey columns = TNFR^−/−, light grey columns = TBK1^−/−/TNFR^−/−, Student’s t test *P < 0.05, **P < 0.01, and ***P < 0.001 in comparison to untreated controls, ^# P < 0.05, ^## P < 0.01 significant mean difference between TNFR^−/− and TBK1^−/−/TNFR^−/− mice