Fig. 7

Regulation of MAPK activity in the spinal cord of wild type, TNFR^−/− and TBK1^−/−/TNFR^−/− mice. Western blots showing the activation of SAPK (a), JNK (b), ERK1 (c), and ERK 2 (d) in different genotypes as assessed by Western blot analysis with phospho-specific antibodies. The blots were normalized to the respective total protein levels and additionally against the loading control HSP90 or β-actin. The blots show a representative result, the diagram shows the densitometric analysis of three independent experiments (n = 3 mice/group); dark columns = control; bright columns = 30 min formalin. Student’s t test *P < 0.05 and **P < 0.01 significant mean difference between untreated and formalin–injected mice. ^# P < 0.05 and ^## P < 0.01 significant mean difference between TNFR^−/− and TBK1^−/−/TNFR^−/− mice