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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Lenalidomide reduces microglial activation and behavioral deficits in a transgenic model of Parkinson’s disease

Fig. 5

Lenalidomide modulates NF-κB signaling and cytokine expression in mThy1-α-syn tg mice. a Immunoblot analysis of α-syn, NF-κB p65, TNFα, and CX3CL1 (fractalkine) in protein extracts from non-tg and mThy1-α-syn tg mice treated with vehicle, lenalidomide, or thalidomide. Significant results of three to four mice per group are shown. b Densitometric analysis of the levels of the α-syn immunoreactive band in cytosol, normalized by actin levels. c Densitometric analysis of the levels of the α-syn immunoreactive band in the particulate/membrane fraction, normalized by actin levels. d Densitometric analysis of the levels of the NF-κB p65 immunoreactive band, expressed as the ratio between the nuclear and cytosolic fractions. e Densitometric analysis of the levels of the TNFα immunoreactive band in cytosol, normalized by actin levels. f Densitometric analysis of the levels of the fractalkine immunoreactive band in cytosol, normalized by actin levels. Error bars represent ± SEM. *, #p < 0.05; ##p < 0.01; ***p < 0.001

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