Fig. 1

hAECs inhibit T cell responses and allogeneic and xenogeneic proliferation. Proliferative response of human PBMCs (a) or splenocytes from naïve C57BL/6 mice (b) stimulated with anti-CD3/CD28 antibodies in the presence or absence of hAECs at different hAECs to splenocyte ratios (n = 6 performed in triplicate, *P < 0.05, ***P < 0.001 compared to T cells). c Proliferative response of 2D2 splenocytes stimulated with 20 μg MOG35-55 in the presence or absence of hAECs at different ratios (n = 6 performed in triplicate, **P < 0.01, ***P < 0.001 compared to T cells). d Cytokine secretion profile in supernatant from co-cultures of hAECs and 2D2 splenocytes at 1:5 ratio (n = 5, **P < 0.01 ***P < 0.001 compared to T cells). e Proliferative response of C57BL/6 splenocytes co-cultured with hAECs (n = 4 performed in triplicate, ***P < 0.0001 compared to B6 + Bc). f Proliferative response of C57BL/6 splenocytes co-cultured with irradiated Balb/c splenocytes in the absence or presence of hAECs at hAEC to C57BL/6 T cell ratio of 1:5 (n = 4 preformed in triplicate, ***P < 0.0001 compared to B6 + Bc). g Cytokine secretion profile in supernatant from MLR cultures with hAECs. (n = 4, *P < 0.05,**P < 0.01, ***P < 0.001 compared to B6 + Bc)