Fig. 6

Protective properties of EP1 and EP3 antagonists. a The EP1 antagonist SC51089 (SC) (1 μM) showed no protection of OPCs against excitotoxic death. b However, the EP3 antagonist ONO-AE5-599 (AE) shows varying amounts of protection across a range of concentrations ranging from 1–30 μM. The lowest concentration of AE that gave the greatest amount of protection was observed at 3 μM. Dispersed OPCs were treated with KA in the presence or absence of SC or AE and analyzed for survival 144 h later. Similar levels of protection of each agent were also observed at 24 and 48 h after KA (not shown). As a positive control for efficacy of SC, parallel treatment of cortical neurons with 1 μM SC conferred neuroprotection of cultured neurons against NMDA-induced excitotoxicity as observed in our previous study [19] (data not shown). The results shown in this figure are representative of four different experiments