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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Inhibition of NADPH oxidase activation reduces EAE-induced white matter damage in mice

Fig. 8

Post-treatment of apocynin also ameliorates the clinical signs and disease progression of EAE. Clinical disease scores were recorded daily until 30 days after induction. Clinical signs of EAE first appeared on day 15 and reached peak levels on day 22 from the 1st MOG (M) immunization. Apocynin (EAE + apocynin, n = 5) or its vehicle (EAE + vehicle, n = 5) was administered orally from day 18 from the 1st MOG (M) immunization. a Post-treatment of apocynin profoundly reduced the EAE clinical score induced by MOG. Data are mean ± sem (n = 5). *p < 0.05 compared with apocynin-treated group. General linear models verified statistical significant differences in clinical scores by time and treatment and interaction between time and treatment was also noticed. b Post-treatment of apocynin did not exert the incidence rate of EAE induced by MOG. c Post-treatment of apocynin reduced MOG-induced demyelination in the spinal cord of EAE mice. Mice were sacrificed 30 days after MOG injection and sections of spinal cord were evaluated for damaged myelin sheaths using luxol fast blue (LFB). White matter lesion was reflected by reduced LFB staining in the spinal cord. LFB staining of the spinal cord shows extensive demyelination in the MOG-immunized EAE mice. EAE-associated demyelination was significantly reduced in EAE mice treated with apocynin. Scale bar represents 50 μm

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