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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Crosstalk between macrophages and astrocytes affects proliferation, reactive phenotype and inflammatory response, suggesting a role during reactive gliosis following spinal cord injury

Fig. 5

Effects of soluble factors from previously stimulated astrocytes on primary macrophages. a Experimental set-up. Astrocytes were stimulated with conditioned medium from M1 or M2 macrophages or left unstimulated. Following a wash, conditioned medium was then collected from these and incubated with fresh primary macrophages. b, c EdU incorporation assays show medium from astrocytes previously stimulated with M2-conditioned medium has an anti-proliferative effect on both M1 and M2 macrophages following 3 days of incubation. d The anti-proliferative effect is confirmed using MTT assays. c As with M1 macrophages, the proliferation of M2 macrophages is also lower when incubated with M2-stimulated astrocyte medium. e In M1 macrophages, TNFα release following β-glucan stimulation, as measured by ELISA, is also affected, with significantly higher release following incubations with M1-stimulated astrocyte medium, and a significant decrease after incubation with M2-stimulated astrocytes medium

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