Fig. 2

Assessment of microglia proliferation and monocyte production by 72 h after stroke. A representative dot plot shows Ki67 expression by microglia at 72 h after stroke (a). The percentages of Ki67⁺ microglia were quantified (b). BRDU incorporation of myeloid cells was assessed in the brain using flow cytometry. A representative dot plot illustrates that resident microglia had not undergone DNA synthesis by 72 h in the ischemic brain (c). Quantification of BRDU⁺ cells revealed that nearly all monocytes had entered S phase of proliferation (d). Analysis of bone marrow revealed that stroke-induced production of monocytes likely account for BRDU⁺ monocytes in the brain (e, f). For all experiments, N = 5/group. Cell-specific FMO controls were used to determine positive gating. Error bars show mean SEM. Abbreviation: SEM standard error of the mean, SH sham, ST stroke. *p < 0.05; ***p < 0.001