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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Functional differences between microglia and monocytes after ischemic stroke

Fig. 3

Measurement of cytokine production by microglia and monocytes at 24 and 72 h and 7 days following stroke. A representative histogram showing microglia (red) have relatively higher reactive oxygen species levels than monocytes (blue) at 72 h after stroke as measured with dihydrorhodamine (DHR) 123 (a). The mean fluorescence intensity of DHR123 was quantified (b). Representative dot plots showing microglia and monocyte production of TNF (c) and IL-1β (e) at 72 h reveals differential expression patterns after stroke. The respective percentages of cells positive for these cytokines are quantified at 0, 24, and 72 hrs and 7 days (d, f). For all experiments, N = 5–7/group. Cell-specific FMO controls were used to determine positive gating. Error bars show mean SEM. Abbreviation: SEM standard error of the mean, SH sham, ST stroke, MFI mean fluorescence intensity. **p < 0.01; ***p < 0.001

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