Fig. 4

Phagocytic activity of microglia and monocytes at 24 and 72 h and 7 days following stroke. Representative histogram showing a relative increase in side scatter (granularity) properties of microglia at 72 h after stroke (red) compared to sham (blue; a). Mean side scatter intensity of microglia was quantified at different time points after MCAO (N = 5/group; b). Phagocytic activity after stroke was measured by bead assay using flow cytometry (c). The percentages and mean fluorescence intensity (MFI) of bead⁺ cells were quantified at 0, 24, and 72 h and 7 days (N = 6/group; d, e). A representative histogram shows higher expression of the phagocytic marker SIRPα on monocytes (blue) compared to microglia (red) at 72 h in the ischemic hemisphere (f). The MFI of SIRPα⁺ cells are quantified (N = 5/group; g). Cell-specific FMO controls were used to determine positive gating. Error bars show mean SEM. Abbreviation: SEM standard error of the mean, SH sham, ST stroke, SSC side scatter, YG yellow green, a.u.i. arbitrary units of intensity