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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Neuronal self-injury mediated by IL-1β and MMP-9 in a cerebral palsy model of severe neonatal encephalopathy induced by immune activation plus hypoxia-ischemia

Fig. 2

Effect of IL-1Ra on IL-1β in LPS+HI-exposed brains. IL-1Ra decreased titers (ELISA) of IL-1β (pg/mg) at 24 and 48 h following LPS+HI exposures (a). IL-1Ra decreased IL-1β relative IHC expressions in the right frontoparietal neocortex, white matter (WM), and striatum at 4 h after LPS+HI exposures (b). IL-1Ra prevented IHC staining of IL-1β in the neocortex of LPS+HI-exposed pups at 4 h post-HI (c). IL-1Ra prevented neuronal IL-1β mRNA expression (arrowhead) in the neocortex 1 h after LPS+HI as detected by in situ hybridization (d). IL-1Ra decreased relative expression of IL-1β mRNA in right frontoparietal cortical neurons 1 h post LPS+HI (e). IF double staining of NeuN+IL-1Ra+ neurons at 4 h post-HI in LPS+HI+IL-1Ra condition (f). Error bars represent means ± SEM, scale bar = 10 μm, four to six animals per conditions, unpaired t test with Welch correction, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. Abbreviations: WM white matter

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