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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Neuronal self-injury mediated by IL-1β and MMP-9 in a cerebral palsy model of severe neonatal encephalopathy induced by immune activation plus hypoxia-ischemia

Fig. 8

Effect of IL-1Ra on MMP-9 expression in LPS+HI-exposed brains. IL-1Ra decreased the titer of total MMP-9 (ng/mg) measured by ELISA within the right cerebral hemisphere at 48 h after exposure to LPS+HI (a). This decrease was confirmed by immunostaining of total MMP-9 expression at 48 h in LPS+HI ± IL-1Ra-exposed brains in gray and white matters (b). The proportion of double positive NeuN+MMP-9+ among NeuN+ neurons (d) from the frontoparietal neocortex of the right hemisphere at 48 h after LPS+HI was not affected by IL-1Ra exposures (c). Decreased right hemisphere percentage of tissue loss in LPS+HI-exposed forebrains treated by IL-1Ra or by SB-3CT at P20 (e). SB3CT prevented the extent of brain lesions after LPS+HI exposure in the same measure as IL-1Ra (f). Core of the lesion was indicated by asterisks. Error bars represent means ± SEM, scale bar = 10 μm, four to six animals per conditions, unpaired t test with Welch correction, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001. Abbreviations: MMP9 matrix metallo-protease 9, NeuN neuronal nuclei, WM white matter

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