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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Immunoglobulins stimulate cultured Schwann cell maturation and promote their potential to induce axonal outgrowth

Fig. 6

IVIG dependent expression and secretion of IL-18 and Mcp-1. a, c, e Two days after transfection, differentiating (diff.) and non-differentiating (non-diff.) Schwann cells were stimulated for up to 7 days (7d) with 20 mg/ml dialysed IVIG or control buffer (ctrl) with one medium exchange at day 4 (4d). At day 7, quantitative RT-PCR revealed a significant upregulation of IL-18 gene expression in non-differentiating cells (a) but not in differentiating cells. However, ELISA determination revealed elevated secreted IL-18 protein levels at both time points, 4d and 7d, after IVIG stimulation (c, e). b, d Similarly, induced IL-18 gene and protein expression levels could be observed when naive (non-transfected and therefore non-differentiating) Schwann cells were IVIG stimulated as shown on time points 1d, 4d, 7d, and 9d. Of note, 4d medium was collected before the medium change. f Mcp-1 gene expression levels were also upregulated after 4d, 7d, and 9d of IVIG stimulation. GAPDH was used as reference gene (a, b, f). t test (ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001). Error bars represent SEM. n = 7 for a, n = 3 for b, f, n = 3 for c, d, e

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