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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure

Fig. 2

Blockade of A2AR prevents microglia morphological changes induced by LPS or EHP. Retinal organotypic cultures were pretreated with the A2AR antagonist SCH 58261 (50 nM) and then challenged with LPS (3 μg/mL) or EHP (+70 mmHg) for 4 h. a Organotypic retinal cultures were immunostained for CD11b (microglia marker; red) and imaged in the GCL using a confocal microscope. Nuclei were stained with DAPI (blue). Representative images obtained from four to five independent experiments. b the circularity index, c number of branches per cell, d number of process endpoints per cell and e the maximum branch length (μm) per cell were calculated for the different experimental conditions. The bar graphs present data as mean ± SEM of four to five independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001, different from control; ++ P < 0.01 and +++ P < 0.001, different from LPS; # P < 0.05 and ## P < 0.01, different from EHP; Kruskal-Wallis test, followed by Dunn’s multiple comparison test

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