Fig. 4
A2AR blockade partially inhibits the inflammatory response induced by LPS or EHP. Retinal organotypic cultures were pretreated with SCH 58261 (50 nM) and then challenged with LPS (3 μg/mL) or EHP (+70 mmHg) for 4 h. a Effects of A2AR blockade in the LPS- or EHP-induced mRNA expression of pro-inflammatory cytokines IL-1β and TNF were assessed by qPCR. Results are presented as fold change of the control, from six to thirteen independent experiments. b The release of IL-1β and TNF to the culture medium was quantified by ELISA. To evaluate the role of endogenous adenosine, the cultures were pretreated with adenosine deaminase (ADA; 1 U/mL). Results are expressed in picogrammes per milliliter and are mean ± SEM of five to ten independent experiments. **P < 0.01, ***P < 0.001 and ****P < 0.0001, different from control; + P < 0.05, and ++ P < 0.01, different from LPS; # P < 0.05 and ## P < 0.01, different from EHP; Kruskal-Wallis test, followed by Dunn’s multiple comparison test