Fig. 5
Blockade of A2AR and of TNF and IL-1 β prevents RGC death induced by LPS or EHP. Retinal organotypic cultures were pretreated with SCH 58261 (50 nM) or with anti-TNF and anti-IL-1β neutralizing antibodies and then challenged with LPS (3 μg/mL) or EHP (+70 mmHg) for 24 h. Rabbit and goat IgGs were used as control for the neutralization experiments. a Organotypic retinal cultures were immunostained for Brn3a (RGC marker, red) after treatment with SCH 58261 prior to challenge. Nuclei were stained with DAPI (blue). b Immunostaining with Brn3a (red) after treatment with neutralizing antibodies prior to challenge. Nuclei were stained with DAPI (blue). Representative images are depicted. c Surviving RGCs are presented as the number of Brn3a-immunoreactive cells per field and are mean ± SEM of five to seven independent experiments. *P < 0.05 and **P < 0.01, different from control; + P < 0.01 and ++ P < 0.01, different from LPS; # P < 0.05 and ## P < 0.01, different from EHP; Kruskal-Wallis test, followed by Dunn’s multiple comparison test