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Table 1 Primary and secondary antibodies used in immunohistochemistry

From: Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure

 

Supplier

Host

Dilution

Primary antibodies

Anti-A2AR

Santa Cruz Biotechnology

Goat

1:100

Anti-CD11b

AbD Serotec

Mouse

1:250

Anti-iNOS

BD Biosciences

Rabbit

1:200

Anti-Brn3a

Chemicon

Mouse

1:500

Secondary antibodies

Alexa Fluor anti-mouse 568

Life Technologies

Donkey

1:200

Alexa Fluor anti-goat 488

Life Technologies

Rabbit

1:200

Alexa Fluor anti-rabbit 488

Life Technologies

Goat

1:200