Table 2 Primers used in qPCR and RT-PCR
Gene | GenBank number | Forward | Reverse | Amplicon size (bp) |
|---|---|---|---|---|
Adora2A | NM_053294 | 5′ - GGCTATCTCTGACCAACA - 3′ | 3′ - TGGCTTGACATCTCTAATCT - 5′ | 106 |
TNF | NM_012675 | 5′ - CCCAATCTGTGTCCTTCT - 3′ | 3′ - TTCTGAGCATCGTAGTTGT - 5′ | 90 |
IL-1β | NM_031512 | 5′ - ATAGAAGTCAAGACCAAAGTG - 3′ | 3′ - GACCATTGCTGTTTCCTAG - 5′ | 109 |
Nos II | NM_012611 | 5′ - AGAGACAGAAGTGCGATC - 3′ | 3′ - AGAGATTCAGTAGTCCACAATA - 5′ | 96 |
hprt | NM_012583 | 5′ - ATGGGAGGCCATCACATTGT- 3′ | 3′ - ATGTAATCCAGCAGGTCAGCAA - 5′ | 76 |
actb | NM_031144 | 5′ - GCTCCTCCTGAGCGCAAG - 3′ | 3′ - CATCTGCTGGAAGGTGGACA - 5′ | 75 |